What is Drabkin solution?
Drabkin′s reagent is used in measuring haemoglobin from blood samples. It comprises potassium ferricyanide, potassium cyanide and potassium dihydrogen phosphate as components. It is a reliable method and can measure all haemoglobin forms except sulphaemoglobin.
What is Cyanmethemoglobin method?
Cyanmethemoglobin method This is the internationally recommended method for determining hemoglobin. Principle: Blood is diluted in a solution containing potassium cyanide and potassium ferricyanide. The latter converts Hb to methemoglobin which is converted to cyanmethemoglobin (HiCN) by potassium cyanide.
What is the other name for the Cyanmethemoglobin method?
Cyanmethemoglobin (Hemoglobin-Cyanide) Method for Estimation of Hemoglobin.
What is Drabkin’s reagent used for?
Drabkin’s Reagent is used for the quantitative, colorimetric determination of hemoglobin concentration in whole blood at 540 nm. Drabkin’s Solution reacts with all forms of hemoglobin except sulfhemoglobin, a pigment that normally occurs in only minute concentrations in blood.
What is the color of Cyanmethemoglobin?
The colour of cyanmethemoglobin is read at 530 nm (4). The DD is judged as a precise, reliable, specific and sensitive method. However, the major disadvantage of this method is the need for accurate pipetting and problem of transporting blood samples for colorimetric reading.
What is Tallquist method?
The Tallquist method of measuring haemoglobin is used to compare a small piece of Tallquist paper that has been saturated with a sample of blood with a Tallquist colour chart.
What is Cyanohemoglobin?
“Although cyanide has a primary affinity for ferric (Fe3+) iron, a small amount may bind to the ferrous (Fe2+) iron of hemoglobin, forming cyanohemoglobin, which is unable to transport oxygen, thereby further exacerbating tissue hypoxia.”
Why is Cyanmethemoglobin method preferred over other methods?
The haemoglobin is estimated with the help of cyanmethemoglobin curve. The advantages of this method are i) error due to subjective visual matching is avoided as spectrophotometer is used and hence reading is precise and reliable, ii) measures all forms of haemoglobin except sulphaemoglobin.
What is the principle of Hemoglobinometer?
The Digital Hemoglobinometer System is based on the principle of reflectance photometry. Capillary, venous, or arterial whole-blood sample can be used for the hemoglobin estimation with the requirement of only 8 ml of blood sample.